We are looking for multiple experienced NMR spectroscopists to study protein kinases. The NMR scientists will join an inter-disciplinary Blue Sky Initiative on ‘Seeing the Invisible in Protein Kinases,’ which is supported by $35M in institutional funding by St. Jude Children’s Research Hospital. The project will extend the ultra-high field NMR spectroscopy and biochemistry approaches developed recently by the Kalodimos lab (Science 2020, 370, eabc2754) to study the human kinome. This is a large-scale project that aims to detect and structurally characterize the distinct conformational states populated by kinases, and to dissect the mechanisms of allosteric regulation and those underpinning oncogenic and drug-resistance mutations. The unparalleled NMR center at St. Jude includes three 600 MHz, two 700 MHz, four 800 MHz, 850 MHz, and the first 1.1 GHz. We expect this initiative to have a profound impact on the field, transforming our understanding of protein kinases.
Preferred skills and experiences
Extensive experience in applying NMR techniques in characterization of the structure and dynamics of large proteins, including backbone and methyl assignment methodologies
techniques in characterization of the structure and dynamics of large proteins, including backbone and methyl assignment methodologies Experience in using both automated and manual methods for assignment of both backbone and methyl groups
Experience in performing characterization of protein dynamics using CEST
Proficient in data processing using NMRPipe
Experienced in NMR structure calculation and refinement
structure calculation and refinement Independently motivated, detail oriented and good problem-solving ability
Excellent organizational skills, with an ability to embrace change and multi-task in an extremely fast-paced environment
Excellent communications skills
St. Jude Children’s Research Hospital offers a remarkable opportunity to perform cutting edge research with outstanding institutional support and exceptional shared core facilities in an environment of open collaboration, educational advancement and training opportunities. St. Jude is consistently ranked on Fortune Magazine’s and Glassdoor’s ‘100 Best Places to Work’ for companies with 1,000 or more employees. We are located in the heart of Memphis, Tennessee, a vibrant and friendly city at the historic American crossroads of music, trade, food, and culture. Mild winters and beautiful green outdoor spaces are part of the reasons why Forbes recently named Memphis the 4th happiest city in which to work. Cost of living and high quality of life are other attractions; Money magazine listed Memphis as one of the least expensive cities in which to raise a child. St. Jude facilities and salaries are highly competitive.