The Department of Biosciences and Nutrition performs research and education in several areas of medical science including aging, Alzheimer, bioorganic chemistry, biomaterial, molecular endocrinology, cancer biology, functional genomics, systems biology, epigenetics, structural biochemistry, immunology, and nutrition. It offers an excellent international research and working environment, including around 250 scientists, students, administrative and technical personnel. The Department resides in Neo at Campus Flemingsberg, a biomedical research building, aimed at being a creative and open environment that enables meetings, synergies, and exploration of areas of mutual interest across disciplines.Do you want to contribute to the development of a novel biomaterial?To be a doctoral student means to devote oneself to a research project under supervision of experienced researchers and following an individual study plan. For a doctoral degree, the equivalent of four years of full-time doctoral education is required.The research groupOur group has developed the first biomimetic method to spin artificial spider silk fibers and succeeded in establishing a production and purification method of recombinant silk proteins that results in unprecedented protein yields (Andersson et al. Nat Chem Biol. 2017; Schmuck et al. Mater Today. 2021). Furthermore, we have sequenced a whole spider genome of outstanding quality and have analyzed the transcriptome of the silk glands. Our research group consists of two PhD students, two technicians, three postdocs and one researcher and is funded by the European Research Council, Swedish Research Council, FORMAS and Center for Innovative Medicine. The research group is located at Karolinska Institutet, campus Flemingsberg, and at SLU in Uppsala.The doctoral student project and the duties of the doctoral studentWe will employ an interdisciplinary approach to further develop the properties of our biomimetic artificial spider silk fibers. In this project we will use analysis of the spider genome and protein engineering to identify novel protein motifs to be expressed and purified from bacterial hosts, spun into fibers and characterized. We will also use state-of-the-art sequencing methods to build a spatially resolved map of gene expression in the silk glands.The successful candidate is expected to have a profound interest in biotechnology and protein biochemistry, enjoy practical lab work as well as bioinformatic analyses. You are expected to independently but with support of the supervisors and fellow group members, generate and analyze data. The project is focused on bioinformatic analyses, protein engineering, recombinant expression of silk proteins and functionalized variants thereof in prokaryotic hosts, biochemical and biophysical characterization of the produced proteins and formulation of different materials and mechanical, structural, and functional characterization. The candidate will supervise master students in the lab. We work in collaboration with other groups and in an interdisciplinary way, which demands the applicant to be independent, creative, have a positive attitude and be collaborative. The candidate will work primarily at KI campus Flemingsberg but is also expected to travel to and work in the lab at SLU in Uppsala.What do we offer? A creative and inspiring environment full of expertise and curiosity. Karolinska Institutet is one of the world’s leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. As a doctoral student you are offered an individual research project, a well-educated supervisor, a vast range of elective courses and the opportunity to work in a leading research group. Karolinska Institutet collaborates with prominent universities from all around the world, which ensures opportunities for international exchanges. You will be employed on a doctoral studentship which means that you receive a contractual salary. Employees also have access to our modern gym for free and receive reimbursements for medical care.Eligibility requirements for doctoral educationIn order to participate in the selection for a doctoral position, you must meet the following general (A) and specific (B) eligibility requirements at latest by the application deadline.It is your responsibility to certify eligibility by following the instructions on the web page Entry requirements (eligibility) for doctoral education.
https://www.nature.com/naturecareers/job/graduate-student-in-next-generation-sequencing-methods-for-the-development-of-a-novel-biomaterial-karolinska-institutet-doctoral-positions-750416
Graduate student in next generation sequencing methods for the development of a novel biomaterial
