We are looking for experienced biochemists to aid in structural studies of protein kinases. The Lead Researcher will join an inter-disciplinary Blue Sky Initiative on ‘Seeing the Invisible in Protein Kinases’, which is supported by $35M in institutional funding by St. Jude Children’s Research Hospital. The project will extend the ultra-high field NMR spectroscopy and biochemistry approaches developed recently by the Kalodimos lab (Science 2020, 370, eabc2754) to study the human kinome. This is a large-scale project that aims to detect and structurally characterize the distinct conformational states populated by kinases, and to dissect the mechanisms of allosteric regulation and those underpinning oncogenic and drug-resistance mutations. We expect this initiative to have a profound impact on the field, transforming our understanding of protein kinases.
Preferred skills and experience:
Expertise in advanced molecular cloning techniques.
Experience generating chimeric recombinant sequences, site-directed mutagenesis, random mutagenesis, codon optimization, and/or gene synthesis is highly desired.
Strong background in protein biochemistry, protein purification (using affinity and traditional chromatographic techniques), and biophysical characterization.
Hands-on experience with instrumentation essential for protein purification and characterization, especially BioRad/ AKTA FPLC , SDS PAGE and Western Blot.
, and Western Blot. Work in an independent manner to perform protein analysis including (but not limited to) CE SDS , ITC , SPR , U/ HPLC , DSF , and cell based functional assays.
, , , U/ , , and cell based functional assays. Excellent oral, presentation and written communication skills.
St. Jude Children’s Research Hospital offers a remarkable opportunity to perform cutting edge research with outstanding institutional support and exceptional shared core facilities in an environment of open collaboration, educational advancement and training opportunities. St. Jude is consistently ranked on Fortune Magazine’s and Glassdoor’s ‘100 Best Places to Work’ for companies with 1,000 or more employees. We are located in the heart of Memphis, Tennessee, a vibrant and friendly city at the historic American crossroads of music, trade, food, and culture. Mild winters and beautiful green outdoor spaces are part of the reasons why Forbes recently named Memphis the 4th happiest city in which to work. Cost of living and high quality of life are other attractions; Money magazine listed Memphis as one of the least expensive cities in which to raise a child. St. Jude facilities and salaries are highly competitive.
https://www.nature.com/naturecareers/job/lead-researcherbiochemist-blue-sky-initiative-st-jude-childrens-research-hospital-st-jude-745657
